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An alternate technique for isolation of Toxocara canis excretory-secretory antigens BJPS
Colli,Cristiane Maria; Elefant,Guita Rubinsky; Paludo,Marcia Liz; Guilherme,Ana Lúcia Falavigna.
The aim of the present study was to test the effectiveness of a sausage-casing membrane for dialysis of Toxocara excretory-secretory antigens (TES). The protein concentrated by the tested membrane was compared with that obtained using a Sigma commercial membrane, as were the protein fractions found by polyacrylamide gel electrophoresis. Standard positive and negative serum samples were evaluated in an ELISA immunoassay, and equivalent data were obtained in all steps, indicating that the sausage-casing membrane is efficient, besides being less expensive to process.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxocara canis/excretory-secretory antigens; Antigens/isolation; Dialysis.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100015
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Clinical features of patients with type 2 diabetes mellitus and hepatitis C infection BJMBR
Greca,L.F.; Pinto,L.C.; Rados,D.R.; Canani,L.H.; Gross,J.L..
The objective of the present cross-sectional study was to assess the prevalence and the clinical and laboratory features of hepatitis C virus (HCV)-positive patients with type 2 diabetes mellitus (DM) attending either an outpatient clinic or hemodialysis units. Serologic-HCV testing was performed in 489 type 2 DM patients (303 outpatients and 186 on dialysis). A structured assessment of clinical, laboratory and DM-related complications was performed and the patients were then compared according to HCV infection status. Mean patient age was 60 years; HCV positivity (HCV+) was observed in 39 of 303 (12.9%) outpatients and in 34 of 186 (18.7%) dialysis patients. Among HCV+ patients, 32 were men (43.8%). HCV+ patients had higher serum levels of aspartate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Type 2 diabetes; Hepatitis C; Dialysis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000300015
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Construction and use of a dialysis chamber for investigating in situ the toxicity of heavy metals on bacteria. ArchiMer
Tan, T.
Teflon dialysis sampling chambers have been employed to study the toxicity of ZnCl sub(2) on a cadmium-sensitive bacterium, Acinetobacter) CS 13, in an aquarium with running seawater. A concentration of 10 mg Zn super(++) l super(-1) did not influence the cell densities in the chambers, but cell densities decreased at zinc concentrations of 15 and 20 mg l super(-1). In seawater with 20 mg Zn super(++) lu-1 the bacteria accumulated zinc from 827 to 2813 mu g g super(-1) cells dry wt after 1 d. After 2 d the zinc concentration increased to 4625 mu g g super(-1) dry wt. Three chambers were filled with Acinetobacter CS 13 cells added to sterile sediment at a concentration of 1 x 10 super(8) cells ml super(-1) wet sediment. To test the security, these chambers...
Tipo: Text Palavras-chave: Acinetobacter; Bacteria; Laboratory culture; Tests; Zinc; Dialysis; Toxicity; Heavy metals.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1012.pdf
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Cross-transmission of vancomycin-resistant Enterococcus in patients undergoing dialysis and kidney transplant BJMBR
Fram,D.; Castrucci,F.M.; Taminato,M.; Godoy-Martinez,P.; Freitas,M.C.S.; Belasco,A.; Sesso,R.; Pacheco-Silva,A.; Pignatari,A.C.; Barbosa,D..
The objective of this study was to investigate the occurrence of vancomycin-resistant Enterococcus (VRE) cross-transmission between two patient groups (long-term dialysis and kidney transplant patients). Molecular typing, by automated ribotyping with the RiboPrinter Microbial Characterization System (Qualicon, USA), was used to analyze VRE isolates from 31 fecal samples of 320 dialysis patients and 38 fecal samples of 280 kidney transplant patients. Clonal spread of E. faecalis and E. casseliflavus was observed intragroup, but not between the two groups of patients. In turn, transmission of E. gallinarum and E. faecium between the groups was suggested by the finding of vancomycin-resistant isolates belonging to the same ribogroup in both dialysis and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vancomycin-resistant Enterococcus; Transmission; Dialysis; Kidney transplant.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100016
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Cytokine accumulation in osteitis fibrosa of renal osteodystrophy BJMBR
Duarte,M.E.L.; Carvalho,E.F.; Cruz,E.A.S.; Lucena,S.B.G.; Andress,D.L..
Bone marrow fibrosis occurs in association with a number of pathological states. Despite the extensive fibrosis that sometimes characterizes renal osteodystrophy, little is known about the factors that contribute to marrow accumulation of fibrous tissue. Because circulating cytokines are elevated in uremia, possibly in response to elevated parathyroid hormone levels, we have examined bone biopsies from 21 patients with end-stage renal disease and secondary hyperparathyroidism. Bone sections were stained with antibodies to human interleukin-1alpha (IL-1alpha), IL-6, IL-11, tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor-ß (TGF-ß) using an undecalcified plastic embedding method. Intense staining for IL-1alpha, IL-6, TNF-alpha and TGF-ß...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytokines; Osteitis fibrosa; Renal osteodystrophy; Chronic renal failure; Dialysis; Secondary hyperparathyroidism.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100004
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Detection of microorganisms, endotoxins and aluminum in mobile dialysis services Biological Sciences
Jesus, Priscila Rodrigues de; Ferreira, Joana Angélica Barbosa; Freitas, Hércules Rezende; Abrantes, Shirley de Mello Pereira; Marin, Victor Augustus.
 Hemodialysis water and dialysates are fundamental in the treatment of kidney disease. During just one hemodialysis session, 120 liters of dialysate are consumed. Thus, it is essential that the parameters of chemical and microbiological quality of the fluids used in dialysis systems are carefully observed. In this study, water samples were collected at 12 hospitals in the state of Rio de Janeiro. The samples were obtained at three points of fluid reservoirs: pre-, post-osmosis and dialysis solution. After collection, colony forming units (CFU), total coliforms and Escherichia coli 100 mL-1 were quantified. Later, isolated colonies and endotoxin content were identified by biochemical assays. Data about total aluminum levels per sample (mg L-1) were also...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dialysis; Hemodialysis units; Dialysis solutions..
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31779
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Hepatitis C risk factor for patients submitted to dialysis BJID
Baldessar,Maria Zélia; Bettiol,Jane; Foppa,Fabrício; Oliveira,Lúcia Helena das Chagas.
This article reports the results of the research which has evaluated the prevalence and factors associated to the presence of Hepatitis C in patients submitted to dialysis at the Clinica de Doenças Renais (Clinic of Renal Diseases) in Tubarao city (CRDT), Santa Catarina State, Brazil, in the period between January 1st, 2004 to December 31st in the same year. The prevalence of 16.8% of Hepatitis C in the studied population and the time-length of dialysis as significative risk factor have become evident. The non-correlation of seropositivity of the followings factors is also indicated: age, gender, base diseases, infrastructures, the type of clinic machines, the type of dialyser, used membranes, the machine sterilisation and substances for this process as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dialysis; Hepatitis C; Nosocomial infection.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100005
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Intermittent hemodialysis in dogs with chronic kidney disease stage III Ciência Rural
Melchert,Alessandra; Geraldes,Silvano Salgueiro; Vieira,André Nanny Le Sueur; Takahira,Regina Kiomi; Ramos,Paulo Roberto Rodrigues; Barretti,Pasqual; Padovani,Carlos Roberto; Sant’Ana,Paula Bilbau; Ribeiro,José Francisco Antunes; Guimarães-Okamoto,Priscylla Tatiana Chalfun.
ABSTRACT: Intermittent hemodialysis (IHD) is a form of renal replacement that is used in veterinary medicine for cases involving drug removal, electrolyte imbalance, acute kidney injury, and chronic kidney disease (CKD). The aim of the present study was to verify the efficacy of IHD in dogs with CKD staged at grade III and to evaluate the effect of IHD on quality of life. Twelve dogs with CKD at stage III met the inclusion criteria and were divided equally into two groups. The control group (n=6) received only clinical treatment and intravenous fluid therapy, and the hemodialysis group (n=6) received clinical and IHD treatments. Blood samples were collected before and after treatments in both groups. We evaluated complications and clinical parameters of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dialysis; CKD; Kidney; Nephrology; Dog.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001000601
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Prognostic factors in patients undergoing early-start peritoneal dialysis within 24 h after catheter insertion BJMBR
Jiang,Hong Ying; Huang,Dan Ju; Bai,Yi Hua; Li,Ji Sai; Pi,Hong Yan; Chen,Jing; Li,Luo Hua; Li,Jing.
This study aimed to investigate the clinical characteristics, prognosis, and factors for survival of patients who underwent early-start peritoneal dialysis (PD) within 24 h after catheter insertion three years after PD. This study was conducted from January 1, 2013 to December 31, 2017. All adult patients who were diagnosed with end-stage renal disease (ESRD) and underwent PD for the first time within 24 h after catheter insertion in our hospital were included. All patients with PD were followed-up until they withdrew from PD, switching to hemodialysis, were transferred to other medical centers, underwent renal transplantation, died or were lost to follow-up, or continued to undergo dialysis until the end of the study period. The follow-up observation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peritoneal dialysis; Early-start peritoneal dialysis; Survival rates; End stage renal disease; Dialysis.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300608
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Renal involvement in leptospirosis: new insights into pathophysiology and treatment BJID
Cerqueira,Thaís Bandeira; Athanazio,Daniel Abensur; Spichler,Anne Stambovsky; Seguro,Antônio Carlos.
Acute renal failure (ARF) is one of the most common complications of leptospirosis although the causal mechanisms are still unclear. Diverse mechanisms are implicated in leptospiral nephropathy and new data supports the role of peculiar ion transport defects. Besides antibiotic therapy, ARF management in leptospirosis requires dialytic therapy which is most efficient when started early. Dialysis is the standard supportive therapy even though recent evidence suggests clinical benefit from alternative treatments such as plasmapheresis and hemofiltration. Renal recovery is achieved soon after clinical improvement. The comprehension of the primary mechanisms of renal dysfunction will be helpful in the development of additional therapeutic tools for improving...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospirosis; Kidney failure; Acute; Physiopathology; Dialysis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300016
Registros recuperados: 10
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